Rv3014c - NAD-dependent DNA ligase ligA

Expression: Most Significant Conditions for Rv3014c

Protein Domains

Gene Information
Locus	Rv3014c
Symbol	ligA
Gene Name	NAD-dependent DNA ligase ligA
Location	3372545 - 3374620 (-)
Species	Mycobacterium tuberculosis H37Rv complete genome.
Length	Gene:2076 bp Protein:692 aa
External Links	Tuberculist Target Gene Information String Protein-Protein Interactions STITCH Chemical-Protein Interactions Search Google Scholar
Orthologs
Orthogroup Number	530
Related Genes	Acel_0694 BL0052 CE1339 cg1401 DIP1077 jk1317 MAV_3868 Mkms_1927 ML1705 Mmcs_1881 MSMEG_2362 MT3094 MUL_1933 Mvan_2112 nfa42650 PPA1626 SAV2748
Transcriptional Regulation
Operons	View gene in operon browser
Regulatory Network	Search for regulators of Rv3014c
Expression Correlation	Genes with Correlated Expression Scatterplot of Gene Expression

Sequence

Proteins

>Rv3014c Transcript 1
MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
SKYDKAVELGVPILDEDGFRRLLADGPASRT*

Genomic Sequence

>Rv3014c
GTGAGCTCCCCAGACGCCGATCAGACCGCTCCCGAGGTGTTGCGGCAGTGGCAGGCACTG
GCCGAGGAGGTGCGTGAGCACCAGTTCCGTTATTACGTGCGGGACGCGCCGATCATCAGC
GACGCGGAATTCGACGAGCTGCTGCGCCGTCTGGAAGCCCTCGAGGAGCAGCATCCCGAG
CTGCGCACGCCCGATTCGCCGACCCAGCTGGTCGGCGGTGCCGGCTTCGCCACGGATTTC
GAGCCCGTCGACCATCTCGAACGAATGCTCAGCCTCGACAACGCGTTCACCGCCGACGAA
CTCGCCGCCTGGGCCGGCCGCATCCATGCCGAGGTCGGAGACGCCGCACATTACCTGTGT
GAGCTCAAGATCGACGGCGTCGCGCTGTCTTTGGTCTACCGCGAGGGACGGCTGACCCGG
GCCTCCACCCGCGGCGACGGGCGCACCGGCGAGGACGTCACCCTGAACGCCCGGACCATC
GCCGACGTTCCCGAACGGCTCACCCCCGGCGACGACTACCCGGTGCCCGAGGTCCTCGAG
GTCCGCGGCGAGGTCTTCTTCCGGCTGGACGACTTCCAGGCGCTCAACGCCAGCCTCGTC
GAGGAGGGCAAGGCGCCGTTCGCCAACCCCCGCAACAGCGCGGCGGGATCGCTGCGCCAG
AAAGACCCGGCGGTCACCGCGCGCCGCCGGCTGCGGATGATCTGCCACGGGCTGGGCCAC
GTGGAGGGCTTTCGCCCGGCCACCCTGCATCAGGCATACCTGGCGTTGCGGGCATGGGGA
CTGCCGGTTTCCGAACACACCACCCTGGCAACCGACCTGGCCGGTGTGCGCGAGCGCATC
GACTACTGGGGCGAGCACCGCCACGAGGTGGACCACGAAATCGACGGCGTGGTGGTCAAA
GTCGACGAGGTGGCGTTGCAGCGCAGGCTGGGTTCCACGTCGCGGGCGCCGCGCTGGGCC
ATCGCCTACAAGTACCCGCCCGAGGAAGCGCAGACCAAGCTGCTCGACATCCGGGTGAAC
GTCGGCCGCACCGGGCGGATCACGCCGTTTGCGTTCATGACGCCGGTGAAGGTGGCCGGG
TCGACGGTGGGACAGGCCACCCTGCACAACGCCTCGGAGATCAAGCGCAAGGGCGTGCTG
ATCGGCGACACCGTGGTGATCCGCAAGGCCGGCGACGTGATCCCCGAGGTGCTGGGACCC
GTCGTCGAACTGCGCGATGGCTCCGAACGCGAATTCATCATGCCCACCACCTGCCCGGAG
TGCGGTTCGCCGTTGGCGCCGGAGAAGGAAGGCGACGCCGACATCCGTTGCCCCAACGCC
CGCGGCTGCCCGGGGCAACTGCGGGAGCGGGTTTTCCACGTCGCCAGCCGCAACGGCCTA
GACATCGAGGTGCTCGGTTACGAGGCGGGTGTGGCGCTCTTGCAGGCGAAGGTGATCGCC
GACGAGGGCGAGCTGTTCGCGCTGACCGAGCGGGACTTGCTGCGCACCGACCTGTTCCGA
ACCAAGGCAGGCGAACTGTCGGCCAACGGCAAACGGCTGCTGGTCAACCTCGACAAGGCC
AAGGCGGCACCGCTGTGGCGGGTGCTGGTGGCGCTGTCCATCCGCCATGTCGGGCCGACG
GCGGCCCGCGCCCTGGCCACCGAGTTCGGCAGCCTTGACGCCATCGCCGCGGCGTCCACC
GACCAGCTGGCCGCCGTCGAGGGGGTGGGGCCGACCATTGCCGCCGCGGTCACCGAGTGG
TTCGCCGTCGACTGGCACCGCGAGATCGTCGACAAGTGGCGGGCCGCCGGGGTGCGAATG
GTCGACGAGCGTGACGAGAGTGTGCCACGCACGCTGGCCGGGCTGACCATCGTGGTCACC
GGCTCGCTGACCGGTTTCTCCCGCGACGACGCCAAGGAGGCGATCGTGGCCCGCGGCGGC
AAGGCCGCCGGCTCGGTGTCGAAGAAGACCAACTATGTCGTCGCCGGAGACTCGCCGGGA
TCCAAATACGACAAGGCGGTGGAGTTGGGGGTGCCGATTCTGGACGAGGATGGGTTCCGG
AGACTGCTGGCCGACGGACCCGCGTCACGAACGTAA

Community Annotations Pending Curatorial Review

Field	Value	Status	Creator	Date
Other	TBPWY:General/ regulation	active	vmizrahi	2012-03-05
Other	TBPWY:General/ regulation	active	vmizrahi	2012-03-05
	Purified and biochemically characterised. Has a low K(m) for NAD(+) (1 mM) and is sensitive to a pyridochromanone inhibitor of NAD(+)-dependent ligases
Other	TBPWY:General/ regulation	active	vmizrahi	2012-03-05
	Novel structure-function relationship elucidated using mutant forms of the enzyme and specific inhibitor identified