Rv1629 - DNA polymerase I polA

Expression: Most Significant Conditions for Rv1629

Protein Domains

Gene Information
Locus	Rv1629
Symbol	polA
Gene Name	DNA polymerase I polA
Location	1830665 - 1833379 (+)
Species	Mycobacterium tuberculosis H37Rv complete genome.
Length	Gene:2715 bp Protein:905 aa
External Links	Tuberculist Target Gene Information String Protein-Protein Interactions STITCH Chemical-Protein Interactions Search Google Scholar
Orthologs
Orthogroup Number	577
Related Genes	Acel_1088 BL0985 CE1455 cg1525 DIP1146 jk0818 MAP1322 MAV_3155 Mkms_3057 ML1381 Mmcs_3011 MSMEG_3839 MT1665 MUL_1611 Mvan_3361 nfa18750 PPA0774 SAV6227
Transcriptional Regulation
Operons	View gene in operon browser
Regulatory Network	Search for regulators of Rv1629
Expression Correlation	Genes with Correlated Expression Scatterplot of Gene Expression

Sequence

Proteins

>Rv1629 Transcript 1
MVTTASAPSEDRAKPTLMLLDGNSLAFRAFYALPAENFKTRGGLTTNAVYGFTAMLINLL
RDEAPTHIAAAFDVSRQTFRLQRYPEYKANRSSTPDEFAGQIDITKEVLGALGITVLSEP
GFEADDLIATLATQAENEGYRVLVVTGDRDALQLVSDDVTVLYPRKGVSELTRFTPEAVV
EKYGLTPRQYPDFAALRGDPSDNLPGIPGVGEKTAAKWIAEYGSLRSLVDNVDAVRGKVG
DALRANLASVVRNRELTDLVRDVPLAQTPDTLRLQPWDRDHIHRLFDDLEFRVLRDRLFD
TLAAAGGPEVDEGFDVRGGALAPGTVRQWLAEHAGDGRRAGLTVVGTHLPHGGDATAMAV
AAADGEGAYLDTATLTPDDDAALAAWLADPAKPKALHEAKAAVHDLAGRGWTLEGVTSDT
ALAAYLVRPGQRSFTLDDLSLRYLRRELRAETPQQQQLSLLDDDDTDAETIQTTILRARA
VIDLADALDAELARIDSTALLGEMELPVQRVLAKMESAGIAVDLPMLTELQSQFGDQIRD
AAEAAYGVIGKQINLGSPKQLQVVLFDELGMPKTKRTKTGYTTDADALQSLFDKTGHPFL
QHLLAHRDVTRLKVTVDGLLQAVAADGRIHTTFNQTIAATGRLSSTEPNLQNIPIRTDAG
RRIRDAFVVGDGYAELMTADYSQIEMRIMAHLSGDEGLIEAFNTGEDLHSFVASRAFGVP
IDEVTGELRRRVKAMSYGLAYGLSAYGLSQQLKISTEEANEQMDAYFARFGGVRDYLRAV
VERARKDGYTSTVLGRRRYLPELDSSNRQVREAAERAALNAPIQGSAADIIKVAMIQVDK
ALNEAQLASRMLLQVHDELLFEIAPGERERVEALVRDKMGGAYPLDVPLEVSVGYGRSWD
AAAH*

Genomic Sequence

>Rv1629
GTGGTGACTACTGCCAGTGCCCCCAGCGAGGATCGAGCCAAGCCGACGCTGATGTTGCTG
GATGGCAATTCGCTGGCGTTTCGGGCGTTCTACGCACTGCCCGCGGAGAACTTCAAGACC
CGCGGCGGGCTGACCACCAACGCCGTCTACGGCTTCACCGCCATGCTGATCAACCTGCTG
CGCGATGAAGCCCCGACGCACATCGCGGCGGCTTTCGACGTGTCCCGGCAGACCTTCCGC
TTGCAACGCTACCCGGAGTACAAGGCCAACCGATCGTCGACCCCCGACGAGTTCGCTGGC
CAGATCGACATCACCAAAGAAGTGCTGGGCGCACTCGGCATCACCGTGCTCTCCGAGCCG
GGGTTCGAGGCCGACGACCTCATCGCCACGCTGGCCACCCAGGCCGAGAACGAGGGCTAC
CGGGTGCTGGTGGTCACCGGGGATCGTGACGCACTGCAACTGGTCAGTGACGATGTGACG
GTGCTCTACCCCCGCAAGGGCGTCAGCGAACTTACGCGCTTCACACCGGAGGCCGTCGTC
GAAAAGTACGGGCTCACCCCTAGGCAGTACCCGGACTTCGCCGCGCTGCGCGGCGACCCC
AGCGATAACCTGCCCGGCATACCCGGGGTGGGGGAGAAGACCGCCGCCAAATGGATCGCC
GAGTACGGCTCGCTGCGGTCACTGGTGGACAACGTTGACGCCGTGCGCGGCAAGGTGGGC
GATGCGCTGCGGGCGAACCTGGCCAGCGTGGTGCGCAACCGTGAGCTCACCGACCTGGTT
CGCGACGTGCCGCTGGCCCAGACCCCGGACACGCTGCGGCTGCAGCCCTGGGATCGCGAC
CACATTCACCGGCTCTTCGACGACCTGGAGTTTCGGGTGTTGCGCGACCGGTTGTTCGAC
ACGTTGGCCGCGGCCGGGGGACCCGAGGTCGACGAGGGGTTCGACGTGCGCGGCGGCGCG
TTGGCGCCCGGCACGGTTAGGCAATGGTTGGCCGAGCACGCCGGCGACGGGCGCCGAGCG
GGCCTGACGGTGGTGGGTACCCATCTGCCGCACGGTGGGGACGCTACCGCTATGGCCGTC
GCCGCCGCCGACGGCGAAGGCGCTTACCTCGATACCGCGACGCTGACGCCCGACGACGAC
GCCGCGTTGGCGGCCTGGCTAGCGGATCCAGCTAAACCCAAAGCCTTGCATGAGGCAAAG
GCGGCCGTTCATGACCTGGCGGGTCGTGGTTGGACCTTGGAGGGCGTCACCTCCGACACC
GCACTGGCGGCCTACCTGGTGCGGCCGGGGCAGCGCAGCTTCACCCTCGACGACCTCTCG
CTGCGCTATCTGCGTCGCGAGCTGCGTGCGGAAACACCGCAGCAGCAACAACTTTCACTG
CTCGATGACGACGATACGGACGCCGAGACCATTCAAACGACGATCCTGCGGGCGCGGGCA
GTCATCGACCTGGCCGACGCGCTGGACGCCGAGTTAGCGCGTATCGACTCCACCGCGCTG
CTGGGGGAGATGGAGCTGCCGGTCCAGCGGGTGCTGGCGAAGATGGAAAGTGCCGGTATC
GCCGTCGACCTGCCCATGTTGACCGAGCTGCAAAGCCAGTTTGGCGACCAGATCCGCGAC
GCCGCCGAGGCCGCCTACGGCGTGATCGGCAAGCAAATCAACCTGGGCTCACCCAAGCAG
CTGCAGGTCGTGCTGTTCGACGAACTGGGCATGCCGAAGACCAAACGCACCAAGACCGGC
TACACCACGGATGCCGACGCGCTGCAGTCGTTGTTCGACAAGACCGGGCATCCGTTTCTG
CAACATCTGCTCGCCCACCGCGACGTCACCCGGCTCAAGGTCACCGTCGACGGGTTGCTC
CAAGCGGTGGCCGCCGACGGCCGCATCCACACCACGTTCAACCAGACGATCGCCGCGACC
GGCCGGCTCTCCTCGACCGAACCCAACCTGCAGAACATCCCGATCCGCACCGACGCGGGC
CGGCGGATCCGGGACGCGTTCGTGGTCGGGGACGGCTACGCCGAGTTGATGACGGCCGAC
TACAGCCAGATCGAGATGCGGATCATGGCGCACCTGTCCGGGGACGAGGGCCTCATCGAG
GCGTTCAACACCGGGGAGGACCTGCATTCGTTCGTCGCGTCCCGGGCGTTCGGCGTGCCC
ATCGACGAGGTCACCGGCGAGCTGCGGCGCCGGGTCAAGGCGATGTCCTACGGGCTGGCT
TACGGGTTGAGCGCCTACGGCCTGTCGCAGCAGTTGAAAATCTCCACCGAGGAAGCCAAC
GAGCAGATGGACGCGTATTTCGCCCGATTCGGCGGGGTGCGCGACTACCTGCGCGCCGTA
GTCGAGCGGGCCCGCAAGGACGGCTACACCTCGACGGTGCTGGGCCGTCGCCGCTACCTG
CCCGAGCTGGACAGCAGCAACCGTCAAGTGCGGGAGGCCGCCGAGCGGGCGGCGCTGAAC
GCGCCGATCCAGGGCAGCGCGGCCGACATCATCAAGGTGGCCATGATCCAGGTCGACAAG
GCGCTCAACGAGGCACAGCTGGCGTCGCGCATGCTGCTGCAGGTCCACGACGAGCTGCTG
TTCGAAATCGCCCCCGGTGAACGCGAGCGGGTCGAGGCCCTGGTGCGCGACAAGATGGGC
GGCGCTTACCCGCTCGACGTCCCGCTGGAGGTGTCGGTGGGCTACGGCCGCAGCTGGGAC
GCGGCGGCGCACTGA

Community Annotations Pending Curatorial Review

Field	Value	Status	Creator	Date
Other	TBPWY:General/ regulation	active	vmizrahi	2012-03-05
	Functional role of a conserved aspartate in the hinge joining the M and N helices investigated. Aspartate in the hinge region is catalytically important and is required for dNTP binding and in the formation of a prepolymerase ternary complex
Other	TBPWY:General/ regulation	active	vmizrahi	2012-03-05
	Recominant enzyme purified and biochemically characterised. Unable to discriminate against ddNTPs and is inhibited by these chain-terminating nucleotide analogues. Role of 9 invariant acidic residues in 5' nuclease domain evaluated. Acidic side chain neutralisation resulted in a >20-fold reduction in activity, with greatest reduction caused by neutralisation of Asp125, Asp148 and Asp150