Rv2215 - pyruvate dehydrogenase E2 component sucB

Expression: Most Significant Conditions for Rv2215

Protein Domains

Gene Information
Locus	Rv2215
Symbol	dlaT
Gene Name	pyruvate dehydrogenase E2 component sucB
Location	2481965 - 2483626 (+)
Species	Mycobacterium tuberculosis H37Rv complete genome.
Length	Gene:1662 bp Protein:554 aa
External Links	Tuberculist Target Gene Information String Protein-Protein Interactions STITCH Chemical-Protein Interactions Search Google Scholar
Orthologs
Orthogroup Number	28992
Related Genes	Acel_0935 CE2098 cg2421 DIP1639 jk0707 MAP1956 MAV_2273 Mkms_3375 ML0861 Mmcs_3313 MSMEG_4283 MT2272 MUL_3575 Mvan_3579 nfa16900 PPA0693 SAV6022
Transcriptional Regulation
Operons	View gene in operon browser
Regulatory Network	Search for regulators of Rv2215
Expression Correlation	Genes with Correlated Expression Scatterplot of Gene Expression

Sequence

Proteins

>Rv2215 Transcript 1
MAFSVQMPALGESVTEGTVTRWLKQEGDTVELDEPLVEVSTDKVDTEIPSPAAGVLTKII
AQEDDTVEVGGELAVIGDAKDAGEAAAPAPEKVPAAQPESKPAPEPPPVQPTSGAPAGGD
AKPVLMPELGESVTEGTVIRWLKKIGDSVQVDEPLVEVSTDKVDTEIPSPVAGVLVSISA
DEDATVPVGGELARIGVAADIGAAPAPKPAPKPVPEPAPTPKAEPAPSPPAAQPAGAAEG
APYVTPLVRKLASENNIDLAGVTGTGVGGRIRKQDVLAAAEQKKRAKAPAPAAQAAAAPA
PKAPPAPAPALAHLRGTTQKASRIRQITANKTRESLQATAQLTQTHEVDMTKIVGLRARA
KAAFAEREGVNLTFLPFFAKAVIDALKIHPNINASYNEDTKEITYYDAEHLGFAVDTEQG
LLSPVIHDAGDLSLAGLARAIADIAARARSGNLKPDELSGGTFTITNIGSQGALFDTPIL
VPPQAAMLGTGAIVKRPRVVVDASGNESIGVRSVCYLPLTYDHRLIDGADAGRFLTTIKH
RLEEGAFEADLGL*

Genomic Sequence

>Rv2215
ATGGCCTTCTCCGTCCAGATGCCGGCACTCGGTGAGAGCGTCACCGAGGGGACGGTTACC
CGCTGGCTCAAACAGGAAGGCGACACGGTCGAACTCGACGAGCCCCTCGTGGAGGTGTCG
ACCGACAAGGTCGACACCGAAATCCCCTCGCCGGCCGCGGGTGTGCTGACCAAGATCATC
GCCCAGGAGGATGACACGGTCGAGGTCGGCGGCGAGCTCGCTGTCATTGGCGACGCCAAG
GATGCCGGCGAGGCCGCGGCCCCGGCACCCGAGAAAGTCCCTGCGGCCCAACCCGAGTCC
AAGCCGGCACCCGAACCACCACCGGTCCAACCGACGTCCGGAGCGCCTGCTGGTGGCGAT
GCCAAGCCGGTGCTGATGCCCGAGCTCGGCGAATCGGTGACCGAGGGGACCGTCATTCGT
TGGCTGAAGAAGATCGGGGATTCGGTTCAGGTTGACGAGCCACTCGTGGAGGTGTCCACC
GACAAGGTGGACACCGAGATCCCGTCCCCGGTGGCTGGGGTCTTGGTCAGTATCAGCGCC
GACGAGGACGCCACGGTGCCCGTCGGCGGCGAGTTGGCCCGGATCGGTGTCGCTGCCGAC
ATCGGCGCCGCGCCCGCCCCCAAGCCCGCACCCAAGCCCGTCCCCGAGCCAGCGCCGACG
CCGAAGGCCGAACCCGCACCATCGCCGCCGGCGGCCCAGCCAGCCGGTGCGGCCGAGGGC
GCACCGTACGTGACGCCGCTGGTGCGAAAGCTGGCGTCGGAAAACAACATCGACCTCGCC
GGGGTGACCGGCACCGGAGTGGGTGGTCGCATCCGCAAACAGGATGTGCTGGCCGCGGCT
GAGCAAAAGAAGCGGGCGAAAGCACCGGCGCCGGCCGCCCAGGCCGCCGCCGCGCCGGCC
CCGAAAGCGCCGCCTGCCCCTGCGCCGGCGTTGGCACATCTACGGGGCACCACCCAGAAG
GCCAGCCGGATTCGTCAGATCACCGCCAACAAGACCCGCGAATCTTTGCAGGCAACGGCA
CAGCTGACACAAACCCATGAGGTCGACATGACCAAGATCGTGGGGCTACGGGCCCGGGCC
AAGGCGGCGTTCGCCGAGCGTGAGGGCGTGAACCTGACCTTCCTGCCGTTCTTCGCCAAG
GCCGTGATCGATGCCCTCAAGATTCACCCGAACATCAACGCTAGCTACAACGAGGACACC
AAGGAGATCACCTACTACGACGCCGAGCACCTAGGATTCGCTGTCGACACCGAGCAGGGC
CTGCTCTCCCCGGTCATCCACGACGCCGGCGATCTGTCACTGGCCGGTCTGGCGCGGGCG
ATCGCCGATATCGCGGCCCGTGCCCGGTCGGGCAACCTGAAACCCGACGAGTTGTCCGGC
GGCACCTTCACCATCACCAACATCGGTAGCCAGGGCGCGTTGTTCGACACCCCGATCCTG
GTTCCGCCGCAGGCCGCCATGCTGGGCACCGGGGCGATCGTCAAGCGGCCGCGGGTGGTC
GTCGATGCCAGCGGCAACGAGTCGATCGGGGTGCGCTCGGTCTGCTACCTCCCGTTGACC
TATGACCATCGGCTCATCGACGGCGCCGACGCCGGACGTTTCCTCACCACGATCAAGCAC
CGCCTCGAAGAGGGAGCGTTCGAGGCCGATTTAGGACTGTGA

Community Annotations Pending Curatorial Review

Field	Value	Status	Creator	Date
Term	TBRXN:PDH pyruvate dehydrogenase - ISS	active	njamshidi	2012-10-05
	previous annotations with sequence based annotations of pdhA, pdhB, pdhC (Rv2497c, Rv2496c,Rv2495c) were found to be incorrect and removed - this is the appropriate annotation for PDH according to PMID: 16045627. J. Tian, R. Bryk et al. Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes. Mol. Microbiol. 2005
Term	TBRXN:PDH pyruvate dehydrogenase - IDA	active	njamshidi	2012-10-05
	previous annotations with sequence based annotations of pdhA, pdhB, pdhC (Rv2497c, Rv2496c,Rv2495c) were found to be incorrect and removed - this is the appropriate annotation for PDH according to PMID: 16045627. J. Tian, R. Bryk et al. Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes. Mol. Microbiol. 2005
Interaction	PhysicalInteraction Rv0462	active	priyadarshinipriyanka2001	2012-10-05
	Affinity purification (Physical interaction) J. Tian, R. Bryk et al. Variant tricarboxylic acid cycle in Mycobacterium tuberculosis: identification of alpha-ketoglutarate decarboxylase. Proc. Natl. Acad. Sci. U.S.A. 2005
Interaction	RegulatedBy Rv1221	active	yamir.moreno	2012-10-05
	Microarrays. mRNA levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using microarray (or macroarray) experiments.. R. Manganelli, MI. Voskuil et al. The Mycobacterium tuberculosis ECF sigma factor sigmaE: role in global gene expression and survival in macrophages. Mol. Microbiol. 2001
Interaction	RegulatedBy Rv3676	active	yamir.moreno	2012-10-05
	E.coli orthology based inference. Orthologous pair regulator-target found in E.coli. G. Balázsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008
Interaction	RegulatedBy Rv3676	active	yamir.moreno	2012-10-05
	E.coli orthology based inference. Orthologous pair regulator-target found in E.coli. authors,M. Madan Babu,SA. Teichmann,L. Aravind Evolutionary dynamics of prokaryotic transcriptional regulatory networks. J. Mol. Biol. 2006
Term	EC:2.3.1.61 Dihydrolipoyllysine-residue succinyltransferase. - NR	active	extern:JZUCKER	2012-03-06
	Inferred from mutant phenotype J. Tian, R. Bryk et al. Mycobacterium tuberculosis appears to lack alpha-ketoglutarate dehydrogenase and encodes pyruvate dehydrogenase in widely separated genes. Mol. Microbiol. 2005
Term	EC:2.3.1.61 Dihydrolipoyllysine-residue succinyltransferase. - NR	active	extern:JZUCKER	2012-03-06
	Traceable author statement to experimental support authors,KY. Rhee,LP. de Carvalho,R. Bryk,S. Ehrt,J. Marrero,SW. Park,D. Schnappinger,A. Venugopal,C. Nathan Central carbon metabolism in Mycobacterium tuberculosis: an unexpected frontier. Trends Microbiol. 2011
Term	EC:2.3.1.12 Dihydrolipoyllysine-residue acetyltransferase. - NR	active	jjmcfadden	2012-03-05
	Inferred from direct assay J. Buglino, KC. Onwueme et al. Crystal structure of PapA5, a phthiocerol dimycocerosyl transferase from Mycobacterium tuberculosis. J. Biol. Chem. 2004