Rv3793 - membrane indolylacetylinositol arabinosyltransferase embC
Protein Domains

Gene Information
LocusRv3793
SymbolembC
Gene Namemembrane indolylacetylinositol arabinosyltransferase embC
Location4239863 - 4243147 (+)
SpeciesMycobacterium tuberculosis H37Rv complete genome.
LengthGene:3285 bp
Protein:1095 aa
External LinksTuberculist
Target Gene Information
String Protein-Protein Interactions
STITCH Chemical-Protein Interactions
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Orthologs
Orthogroup Number14070
Related GenesMAP0228c MAP0229c MAP0232c MAV_0225 MAV_0229 ML0104 ML0106 MT3900 MT3901 MUL_4972 MUL_4973 nfa1920 nfa1930
Transcriptional Regulation
Operons View gene in operon browser
Regulatory Network
Search for regulators of Rv3793
Expression Correlation Genes with Correlated Expression
Scatterplot of Gene Expression

Sequence
Proteins
Genomic Sequence
Community Annotations Pending Curatorial Review
FieldValueStatusCreatorDate
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
authors,DC. Crick,S. Mahapatra,PJ. Brennan Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology 2001
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
TermTBRXN:AFE arabinofuranosyl extension (M tb) - IPIactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
A. Telenti, WJ. Philipp et al. The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol. Nat. Med. 1997
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
authors,DC. Crick,S. Mahapatra,PJ. Brennan Biosynthesis of the arabinogalactan-peptidoglycan complex of Mycobacterium tuberculosis. Glycobiology 2001
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
LJ. Alderwick, M. Seidel et al. Identification of a novel arabinofuranosyltransferase (AftA) involved in cell wall arabinan biosynthesis in Mycobacterium tuberculosis. J. Biol. Chem. 2006
TermTBRXN:AFE arabinofuranosyl extension (M tb) - ISSactivenjamshidi2012-10-05
This is a lumped reaction involving the emb locus (PMID: 9142129), details of the reaction are not well known. Recently it has been shown that the AftA locus 'primes' the araf extension (see AFTA) (PMID: 16595677), other sources imply that extension of ARaf residues ccurs while bound to the polyprenyl core (PMID: 11555614, Cole et al text Tuberculosis and the tubercle bacillus). Also note that this is the target of ethambutol (hence the locus name).
A. Telenti, WJ. Philipp et al. The emb operon, a gene cluster of Mycobacterium tuberculosis involved in resistance to ethambutol. Nat. Med. 1997
InteractionRegulatory Rv1267cactivepriyadarshinipriyanka20012012-10-05
Band Shift
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatory Rv1266cactiveshahanup862012-10-05
Affinity purification (Physical interaction)
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
qRT-PCR. mRNA expression levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using qRT-PCR technique. Electrophoretic mobility shift assays EMSA. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
qRT-PCR. mRNA expression levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using qRT-PCR technique. Electrophoretic mobility shift assays EMSA. Physical binding of the regulator to the regulated promoter proved by using electrophoretic mobility shift assay. .
K. Sharma, M. Gupta et al. Transcriptional control of the mycobacterial embCAB operon by PknH through a regulatory protein, EmbR, in vivo. J. Bacteriol. 2006
InteractionRegulatedBy Rv1267cactiveyamir.moreno2012-10-05
Literature previously reported link (from Balazsi et al. 2008). Traceable author statement to experimental support.
G. Balázsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008
NameDecaprenol phosphoarabinose-dependent arabinosyltransferase involved in the arabinosylation of LAM; inhibition by ethambutolactivemjackson2012-10-05
PIM, LM and LAM biosynthesis
OtherTBPWY:PIM, LM and LAM biosynthesisactivemjackson2012-03-05
Decaprenol phosphoarabinose-dependent arabinosyltransferase involved in the arabinosylation of LAM (phenotypic [mycobacterial recombinant strains])
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005
OtherTBPWY:PIM, LM and LAM biosynthesisactivemjackson2012-03-05
Decaprenol phosphoarabinose-dependent arabinosyltransferase involved in the arabinosylation of LAM (phenotypic [mycobacterial recombinant strains])
authors,N. Zhang,JB. Torrelles,MR. McNeil,VE. Escuyer,KH. Khoo,PJ. Brennan,D. Chatterjee The Emb proteins of mycobacteria direct arabinosylation of lipoarabinomannan and arabinogalactan via an N-terminal recognition region and a C-terminal synthetic region. Mol. Microbiol. 2003
TermEC:2.4.2.34 Indolylacetylinositol arabinosyltransferase. - NRactivejjmcfadden2012-03-05
Inferred from direct assay
S. Berg, J. Starbuck et al. Roles of conserved proline and glycosyltransferase motifs of EmbC in biosynthesis of lipoarabinomannan. J. Biol. Chem. 2005