Rv2988c - 3-isopropylmalate dehydratase large subunit leuC

Expression: Most Significant Conditions for Rv2988c

Protein Domains

Gene Information
Locus	Rv2988c
Symbol	leuC
Gene Name	3-isopropylmalate dehydratase large subunit leuC
Location	3344654 - 3346075 (-)
Species	Mycobacterium tuberculosis H37Rv complete genome.
Length	Gene:1422 bp Protein:474 aa
External Links	Tuberculist Target Gene Information String Protein-Protein Interactions STITCH Chemical-Protein Interactions Search Google Scholar
Orthologs
Orthogroup Number	32722
Related Genes	Acel_1591 BL1262 CE1427 cg1487 DIP1127 jk1221 MAP3026c MAV_3838 Mkms_1967 ML1685 Mmcs_1921 MSMEG_2387 MT3066 MUL_1968 Mvan_2135 nfa42120 PPA1362 PPA1363 SAV2686 SCO5553
Transcriptional Regulation
Operons	View gene in operon browser
Regulatory Network	Search for regulators of Rv2988c
Expression Correlation	Genes with Correlated Expression Scatterplot of Gene Expression

Sequence

Proteins

>Rv2988c Transcript 1
MALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRR
PELTLATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGP
QLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGR
LPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV
APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTN
PGQGVPLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIE
DLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGM
NPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN*

Genomic Sequence

>Rv2988c
ATGGCCTTGCAGACCGGCGAGCCACGCACACTGGCCGAGAAGATTTGGGACGACCACATT
GTGGTATCAGGCGGGGGCTGTGCGCCCGACTTGATCTACATCGATCTGCATCTGGTGCAT
GAGGTCACCAGCCCGCAGGCGTTCGACGGCCTGCGCCTGGCCGGGCGCCGGGTGCGGCGG
CCCGAGCTCACCCTCGCCACCGAGGATCACAACGTGCCCACCGTCGACATCGACCAGCCG
ATCGCCGACCCGGTGTCACGCACCCAGGTGGAGACGTTGCGCCGAAACTGCGCCGAATTC
GGAATCCGGCTGCATTCAATGGGCGATATCGAGCAGGGCATCGTGCACGTCGTCGGACCG
CAATTGGGTCTCACCCAGCCGGGAATGACGATCGTCTGTGGCGATAGCCACACCTCAACC
CACGGCGCATTCGGCGCGTTGGCGATGGGCATCGGTACGTCGGAGGTCGAGCACGTGCTG
GCCACCCAGACGCTGCCGCTGCGGCCGTTCAAGACCATGGCGGTCAACGTCGATGGGCGG
CTGCCCGACGGTGTGTCGGCCAAGGACATCATCCTGGCGTTGATCGCCAAGATCGGCACC
GGCGGCGGCCAGGGGCATGTCATCGAATACCGGGGCAGCGCCATCGAATCGCTGTCCATG
GAAGGCCGGATGACGATCTGCAACATGAGCATCGAAGCCGGCGCTCGTGCGGGCATGGTG
GCTCCGGACGAAACCACCTACGCGTTCTTGCGTGGTCGTCCGCACGCACCCACCGGTGCC
CAGTGGGACACCGCACTCGTCTACTGGCAACGGCTTCGCACCGACGTCGGCGCCGTTTTC
GACACCGAGGTGTATCTCGACGCCGCGTCGTTGAGCCCGTTTGTCACCTGGGGCACCAAC
CCAGGCCAAGGGGTACCGCTGGCGGCCGCGGTGCCAGATCCGCAACTGATGACCGACGAC
GCCGAGCGGCAGGCCGCCGAGAAAGCATTGGCGTACATGGACCTTCGACCCGGAACCGCG
ATGCGCGACATCGCGGTCGACGCCGTGTTCGTCGGGTCGTGTACCAACGGTCGCATTGAA
GATCTGCGGGTGGTCGCCGAAGTGCTGCGTGGCCGCAAGGTGGCCGACGGCGTGCGGATG
CTGATCGTTCCGGGCTCAATGCGGGTACGCGCGCAGGCCGAGGCCGAAGGGCTCGGTGAG
ATCTTCACCGACGCGGGCGCGCAATGGCGGCAGGCGGGATGCTCGATGTGTCTGGGCATG
AATCCCGATCAGCTGGCGTCCGGGGAGCGCTGCGCCGCGACGTCCAACCGCAACTTCGAA
GGGCGGCAGGGCGCGGGCGGCCGCACACATTTGGTGTCCCCAGCAGTGGCGGCCGCCACC
GCGGTTCGCGGCACACTGTCCAGCCCGGCCGATTTGAATTGA

Community Annotations Pending Curatorial Review

Field	Value	Status	Creator	Date
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Structural Analysis M. Karuppasamy, A. Geerlof et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of isopropylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2009
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Structural Analysis N. Koon, CJ. Squire et al. Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 2004
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Structural Analysis MK. Hondalus, S. Bardarov et al. Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis. Infect. Immun. 2000
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Spectrophotometric M. Karuppasamy, A. Geerlof et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the small subunit of isopropylmalate isomerase (Rv2987c) from Mycobacterium tuberculosis. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2009
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Spectrophotometric N. Koon, CJ. Squire et al. Crystal structure of LeuA from Mycobacterium tuberculosis, a key enzyme in leucine biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 2004
Interaction	PhysicalInteraction Rv2987c	active	kholia.trupti	2012-10-05
	Spectrophotometric MK. Hondalus, S. Bardarov et al. Attenuation of and protection induced by a leucine auxotroph of Mycobacterium tuberculosis. Infect. Immun. 2000
Interaction	RegulatedBy Rv3291c	active	yamir.moreno	2012-10-05
	E.coli orthology based inference. Orthologous pair regulator-target found in E.coli. G. Balázsi, AP. Heath et al. The temporal response of the Mycobacterium tuberculosis gene regulatory network during growth arrest. Mol. Syst. Biol. 2008
Interaction	RegulatedBy Rv0491	active	yamir.moreno	2012-10-05
	Microarrays. mRNA levels of regulated element measured and compared between wild-type and trans-element mutation (knockout, over expression etc.) performed by using microarray (or macroarray) experiments.. T. Parish, DA. Smith et al. The senX3-regX3 two-component regulatory system of Mycobacterium tuberculosis is required for virulence. Microbiology (Reading, Engl.) 2003